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KMID : 0363020050350041019
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Journal of Korean Academy of Periodontology
2005 Volume.35 No. 4 p.1019 ~ p.1037
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The Bone regenerative effects of tetracycline blended chitosan membranes on the calvarial critical size defect in Sprague dawley rats
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채경준
김태균/정의원/이수복/정용식/이용근/김창성/채중규/조규성/김종관/최성호
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Abstract
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The major goals of periodontal therapy are the functional regeneration of periodontal
supporting structures already destructed by periodontal disease as well as the reduction of signs
and symptoms of progressive periodontal disease. There have been many efforts to develop
materials and therapeutic methods to promote periodontal wound healing.
There have been increasing interest on the chitosan made by chtin. Chitosan is a derivative of
chitin made by deacetylation of side chains. Chitosan has been widely studied as bone
substitution and membrane material in periodontology. Many experiments using chitosan in
various animal models have proven its beneficial effects.
Tetracycline has been considered for use in the treatment of chronic periodontal disease and
gingivitis.
The aim of this study is to evlauate the osteogenesis of tetracycline blended chitosan
membranes on the calvarial critical size defect in Sprague Dawley rats. An 8mm surgical defect
was produced with a trephine bur in the area of the midsagittal suture. The rats were divided
into five groups: Untreated control group versus four experimental group. Four types of
membranes were made and comparative study was been done. Two types of non-woven
membranes were made by immersing non-woven chitosan into either the tetracycline solution or
chitosan-tetracycline solution. Other two types of sponge membranes were fabricated by immersing chitosan sponge into the tetracycline solution, and subsequent freeze-drying. The
animals were sacrificed at 2 and 8 weeks after surgical procedure. The specimens were examined
by histologic analyses. The results are as follows:
1. Clinically the use of tetracycline blended chitosan membrane showed great healing capacity.
2. The new bone formations of all the experimental group, non-woven and sponge type
membranes were greater than those of control group. But, there was no significant
difference between the experimental groups.
3. Resorption of chitosan membranes were not shown in any groups at 2 weeks and 8 weeks.
These results suggest that the use of tetracycline blended chitosan membrane on the calvarial
defects in rats has significant effect on the regeneration of bone tissue in itself. And it implicate
that tetracycline blended chitosan membrane might be useful for guided tissue regeneration.4)
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KEYWORD
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chitosan, tetracycline, regeneration
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